3/16/2023 0 Comments Lepton rda clone![]() These semi-nested assays may be useful when the standard IGH/ IGK assays fail to detect clonality in histopathologically suspected HLs. In conclusion, tumor clonality was detected in nearly two-thirds of classical HL cases using semi-nested BIOMED-2 IGH/ IGK assays using paraffin tumor sections. The detection rates were not associated with the HL subtypes, CD30-positive cell density, CD20-positive cell density, or Epstein-Barr virus (EBV) positivity. Clonality was detected in 13/58 (22.4%) classical HL cases using the standard IGH/ IGK assays while it was detected in 38/58 (65.5%) cases using semi-nested IGH/ IGK assays. In both IGH and IGK assays, semi-nested assays yielded significantly higher clonality detection rates than the standard assays and re-amplification assays. Employing 58 cases of classical HL, we carried out the standard BIOMED-2, BIOMED-2 followed by BIOMED-2 re-amplification, and BIOMED-2 followed by semi-nested BIOMED-2, all targeting IGH and IGK, using paraffin-embedded tissues. In this study, we devised a novel semi-nested BIOMED-2 IGK assay. We previously described the usefulness of the semi-nested BIOMED-2 IGH assay in B-cell malignancies. The clonality detection rates, however, have not been very high with these methods using paraffin-embedded tumor sections. The BIOMED-2 PCR protocols targeting IGH and IGK genes may be useful for detecting clonality in Hodgkin lymphoma (HL). Han, Shusen Masaki, Ayako Sakamoto, Yuma Takino, Hisashi Murase, Takayuki Iida, Shinsuke Inagaki, Hiroshi Improved clonality detection in Hodgkin lymphoma using a semi-nested modification of the BIOMED-2 PCR assay for IGH and IGK rearrangements: A paraffin-embedded tissue study. These data demonstrate how IL-7R signaling represses Igk germline transcription and provide a general model for STAT5-mediated epigenetic transcriptional repression. Genome-wide analyses revealed a STAT5 tetrameric binding motif associated with transcriptional repression. H3K27me3 modifications were lost after termination of IL-7R–STAT5 signaling and E2A bound Eκi, resulting in acquisition of H3K4me1 and H4Ac. In the absence of Ezh2, IL-7 failed to repress Igk germline transcription. Ezh2 marked H3K27me3 throughout Jκ to Cκ. We demonstrate that STAT5 tetramer bound the Igk intronic enhancer (Eκi), leading to recruitment of the histone methyltransferase Ezh2. By activating the transcription factor STAT5, IL-7R signaling maintains proliferation and represses Igk germline transcription by unknown mechanisms. Clark, Marcus R.ĭuring B lymphopoiesis, Igk recombination requires pre-B cell receptor (pre-BCR) expression and escape from interleukin 7 receptor (IL-7R) signaling. Maienschein-Cline, Mark Bartom, Elizabeth T. Epigenetic repression of the Igk locus by STAT5-mediated Ezh2 recruitment ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |